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Image Search Results
Journal: PLoS Pathogens
Article Title: Phosphodiesterase-4 Inhibition Alters Gene Expression and Improves Isoniazid – Mediated Clearance of Mycobacterium tuberculosis in Rabbit Lungs
doi: 10.1371/journal.ppat.1002262
Figure Lengend Snippet: (A) Level of INH detected in the plasma of rabbits treated with a bolus dose of either 25 or 50 mg/kg of INH by gavage. INH levels were estimated by HPLC-mass spectrometry as explained in the section (B) Effect of different modes of administration of INH on rabbit plasma INH levels. Rabbits were treated with INH either by gavage using a rubber feeding tube, or in drinking water. Plasma INH from treated rabbits was separated from the blood collected at indicated time points, post-administration, to measure the INH concentration by HPLC-mass spectrometry as explained in the Methods section. The horizontal dotted line in (B) represents the optimal level of quantification of INH.
Article Snippet: The concentration of INH in the plasma of Mtb infected and treated rabbits were measured using Sciex API4000 (Applied Biosystems, CA, USA) mass spectrometer coupled to
Techniques: Clinical Proteomics, Mass Spectrometry, Concentration Assay